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Quantity: 100 ul                                                                                         Application: WB

Predicted I Observed M.W.: 157 I 110 kDa                                                 Uniprot ID: Q9SJQ6

Background:

REPRESSOR OF SILENCING1 (ROS1) is a repressor of transcriptional gene silencing. ROS1 functions by demethylating the target promoter DNA. ROS1 interacts physically with RPA2/ROR1. In the ros1 mutants, an increase in methylation is observed in a number of gene promoters. Among the loci affected by ros1, a few (RD29A and At1g76930) are affected in cytosine methylation in all sequence contexts (CpG, CpNpG or CpNpN), although many others are affected primarily in non-CpG contexts.

Other Names:

ATROS1, DEMETER-LIKE 1, DML1, REPRESSOR OF SILENCING1, ROS1

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of arabidopsis thaliana ROS1 (At2g36490). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: