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Predicted | Observed MW: 83 kDa                                                             Uniprot ID: Q33E94 

Application: WB

Quantity:        50 ul RFX4 (M) (R1286-1) Rabbit Polyclonal Antibody &

                      50 ul RFX4 (C) (R1286-2) Rabbit Polyclonal Antibody 

Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:                        

Transcription factor RFX4 (RFX4) is a member of the regulatory factor X gene family, which contains a highly-conserved winged helix DNA binding domain. RFX4 is structurally related to regulatory factors X1, X2, X3, and X5. RFX4 has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1 [provided by RefSeq].

Other Names:

Transcription factor RFX4, Regulatory factor X 4, Testis development protein NYD-SP10

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the middle [RFX4 (M) (R1286-1)] or the C-terminal [RFX4 (C) (R1286-2)] region of human RFX4. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: