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R1937-vp - TRIP4 (vPairTM) Antibodies
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Predicted I Observed M.W.: 66 kDa Uniprot ID: Q15650
Application: WB
Quantity: 50 ul TRIP4 (N) (R1937-1) Rabbit Polyclonal Antibody &
50 ul TRIP4 (C) (R1937-2) Rabbit Polyclonal Antibody
Product Introduction:
vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.
Background:
Thyroid receptor-interacting protein 4 (TRIP4) is a transcriptional co-activator of nuclear receptors, which functions in conjunction with CBP-p300 and SRC-1 and may play an important role in establishing distinct co-activator complexes under different cellular conditions. TRIP4 plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. TRIP4 also acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B.
Other Names:
HsT17391, TRIP-4, TR-interacting protein 4, Thyroid receptor-interacting protein 4, Activating signal cointegrator 1, ASC-1
Source and Purity:
Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [TRIP4 (N) (R1937-1)] or the C-terminal [TRIP4 (C) (R1937-2)] region of human TRIP4. Antibodies were purified by affinity purification using immunogen.
Storage Buffer and Condition:
Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.
Species Specificity:
Human, Mouse
Tested Applications:
WB: 1:1,000-1:3,000 (detect endogenous protein*)
*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.
Product Data: brain, B) mouse thymus; C) human HeLa; D, E) human Jurkat; using 2 independent Abs against 2 distinct regions of human TRIP4 at RT for 2 h. |