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Quantity:        50 ul FANCI (C2) (R0147-2) Rabbit Polyclonal Antibody &

                      50 ul FANCI (N) (R0147-3) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Fanconi anemia, complementation group I (FANCI) is required for maintenance of chromosomal stability.  It plays a role in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links.  In response to DNA damage it is involved in the S phase and G2 phase checkpoints of the cell cycle.  FANCI also promotes FANCD2 ubiquitination and recruitment to DNA repair sites.  Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage and defective DNA repair.  

Other Names:

FLJ10719, KIAA1794, Protein FACI

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the C-terminal [FANCI (C2) (R0147-2)] or the N-terminal [FANCI (N) (R0147-3)] region of human FANCI.  Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications: 

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: