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Quantity: 100 ul                                                                                           Application: WB

Predicted I Observed M.W.: 72 kDa                                                            Uniprot ID: Q9LDX1

Background:

SUPPRESSOR OF GENE SILENCING 3 (SGS3) is required for posttranscriptional gene silencing and natural virus resistance. SGS3 is a member of an 'unknown' protein family. Members of this family have predicted coiled coiled domains suggesting oligomerization and a potential zinc finger domain. SGS3 is involved in the production of trans-acting siRNAs through direct or indirect stabilization of cleavage fragments of the primary ta-siRNA transcript. SGS3 acts before RDR6 in this pathway.

Other Names:

Protein SUPPRESSOR OF GENE SILENCING 3, AtSGS3

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of arabidopsis thaliana SGS3 (At5g23570). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications:

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: