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Quantity: 100 ul                                                                                          Application: WB

Predicted I Observed M.W.: 28 I 34 kDa                                              Uniprot ID: P41238

Background:

C->U-editing enzyme APOBEC-1 belongs to the cytidine and deoxycytidylate deaminase family. APOBEC1 is a catalytic component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. APOBEC1 is also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA. 

Other Names:

C->U-editing enzyme APOBEC-1, Apolipoprotein B mRNA-editing enzyme 1, HEPR

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human APOBEC1. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

Fig 1.  Western blot of total cell extracts
from A) human HeLa; B) human Jurkat;
using anti-APOBEC1 (N3) (R1087-3) at
RT for 2 h.