Products

R3450-2 - JAZ1 (M) Antibody, Rabbit Polyclonal

Catalog No. R3450-2
Product Name JAZ1 (M) Antibody, Rabbit Polyclonal
Price $349
  
 

Quantity: 100 ul                                                                                          Application: WB

Predicted I Observed M.W.: 28 kDa                                                            Uniprot ID: Q9LMA8


Background:

Jasmonate ZIM domain-containing protein 1 (JAZ1) is a repressor of jasmonate responses negatively regulated by the proteasome in an SCF(COI1) E3 ubiquitin-protein ligase complex-dependent manner. Jasmonoyl-isoleucine (JA-Ile) specifically promotes COI1-JAZ1 interaction. JAZ1 interacts with COI1 and inositol pentakisphosphate to form a high-affinity jasmonates co-receptor.

Other Names:

Protein TIFY 10A, Jasmonate ZIM domain-containing protein 1, At1g19180, T29M8.5, ATJAZ1, JAZ, TIFY10A

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the middle region of arabidopsis thaliana JAZ1 (At1g19180). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

 Jaz1 Antibody Western (Abiocode)

Fig 1. A) Western blot analysis of total protein extracts from wild type arabidopsis
leaves, using anti-JAZ1 (M) (R3450-2) at RT for 2 h. B) Same as in A except that
equal amounts of protein extracts from wild type (WT) or JAZ1 mutant (Mut)
arabidopsis leaves were used for Western blot, and the same filter was re-probed
with a non-relevant Ab for loading controls.