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R3518-2 - PHOT2 (M) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB
Predicted I Observed M.W.: 102 I 140 kDa Uniprot ID: P93025
Background:
Phot2 is a protein kinase that acts as a blue light photoreceptor in a signal-transduction pathway for photo-induced movements. Phot2 phosphorylates BLUS1, a kinase involved in stomatal opening. Phot2 mediates calcium spiking of extra- and intracellular origins in response to blue light. Phot2 is involved in hypocotyl phototropism. Phot2 contributes to the chloroplast accumulation in low blue light and mediates their translocation (avoidance response) at high fluence. Regulates stomata opening and photomorphogenesis response of leaf tissue. Phot2 is not involved in hypocotyl elongation inhibition, anthocyanin accumulation or cotyledon opening.
Other Names:
Phototropin-2, Defective in chloroplast avoidance protein 1, Non-phototropic hypocotyl 1-like protein 1, AtKin7, NPH1-like protein 1, CAV1, KIN7, NPL1, At5g58140, K21L19.6, ATPHOT2
Source and Purity:
Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the middle region of arabidopsis thaliana PHOT2 (AT5G58140). Antibodies were purified by affinity purification using immunogen.
Storage Buffer and Condition:
Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.
Species Specificity:
Arabidopsis thaliana
Tested Applications:
WB: 1:500-1:2,000 (detect endogenous protein*)
*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.
Product Data:
Fig 1. Western blot of equal amounts of protein extracts from WT or PHOT2-deficient mutant (Mut) arabidopsis leaves, using anti-PHOT2 (M) (R3518-2) at RT for 2 h. |