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R3453-1 - FRIGIDA (N) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB
Predicted I Observed M.W.: 35 kDa Uniprot ID: Q67Z93 Background: FRIGIDA is a major determinant of natural variation in Arabidopsis flowering time. Dominant alleles of FRIGIDA confer a vernalization requirement causing plants to overwinter vegetatively. Many early flowering accessions carry loss-of-function fri alleles .Twenty distinct haplotypes that contain non-functional FRIGIDA alleles have been identified and the distribution analyzed in over 190 accessions. The common lab strains- Col and Ler each carry loss of function mutations in FRIGIDA. Other Names: Inactive protein FRIGIDA, At4g00650, F6N23.25, FLOWERING LOCUS A, FRI, REDUCED STEM BRANCHING 7, RSB7 Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of arabidopsis thaliana FRIGIDA (AT4G00650). Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Arabidopsis thaliana Tested Applications: WB: 1:500-1:2,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. A) Western blot analysis of total protein extracts from wild type arabidopsis leaves, using anti-FRIGIDA (N) (R3453-1) at RT for 2 h. B) Western blot of protein extract from human 293T cells transfected with the vector control (Vec) or Flag-tagged FRIGIDA (AT4G00650), using the indicated Abs at RT for 2 h. |