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R2925-vp - GNAS (vPairTM) Antibodies
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Predicted I Observed M.W.: 111 I 140 kDa Uniprot ID: Q5JWF2 Application: WB Quantity: 50 ul GNAS (N) (R2925-1) Rabbit Polyclonal Antibody & 50 ul GNAS (C) (R2925-2) Rabbit Polyclonal Antibody Product Introduction: vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS. Background: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(s) protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. XLas isoforms interact with the same set of receptors as Gnas isoforms. Other Names: Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas, Adenylate cyclase-stimulating G alpha protein, Extra large alphas protein, XLalphas, GNAS1 Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [GNAS (N) (R2925-1)] or the C-terminal [GNAS (C) (R2925-2)] region of human GNAS. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human Tested Applications: WB: 1:1,000-1:3,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: A, C) human HeLa, B) human Jurkat, using 2 independent Abs against 2 distinct regions of human GNAS at RT for 2 h. |