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Predicted I Observed M.W.: 62 kDa                                               Uniprot ID: Q92804

Application: WB

Quantity:        50 ul TAF15 (N1) (R2846-1) Rabbit Polyclonal Antibody &

                      50 ul TAF15 (N2) (R2846-2) Rabbit Polyclonal Antibody 

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

TATA-binding protein-associated factor 2N (TAF15) belongs to the RRM TET family. TAF15 is an RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. TAF15 can enter the preinitiation complex together with the RNA polymerase II (Pol II).

Other Names:

TATA-binding protein-associated factor 2N, 68 kDa TATA-binding protein-associated factor, TAF(II)68, TAFII68, RNA-binding protein 56, RBP56, TAF2N

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing 2 distinct N-terminal regions of human TAF15. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity: 

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: