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R2509-1 - NONO (N) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB Predicted I Observed M.W.: 54 kDa Uniprot ID: Q15233 Background: Non-POU domain-containing octamer-binding protein (NONO) is a DNA- and RNA binding protein, involved in several nuclear processes. NONO binds the conventional octamer sequence in double-stranded DNA. NONO also binds single-stranded DNA and RNA at a site independent of the duplex site. Additionally, NONO is involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Other Names: Non-POU domain-containing octamer-binding protein, NonO protein, 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, DNA-binding p52/p100 complex, 52 kDa subunit, NMT55, p54(nrb), p54nrb, NRB54 Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human NONO. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human, Mouse Tested Applications: WB: 1:1,000-1:3,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: brain, B, F) mouse thymus, C, G) human HeLa, D, H) human Jurkat; 2 independent Abs against 2 distinct regions of human NONO at RT for 2 h. |