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Predicted M.W.: 33 kDa                                                                    Uniprot ID: P51911

Application: WB

Quantity:        50 ul CNN1 (N) (R0197-1) Rabbit Polyclonal Antibody &

                      50 ul CNN1 (C) (R0197-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Calponin-1 (CNN1) is a thin filament-associated protein implicated in the regulation and modulation of smooth muscle contraction. CNN1 is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of CNN1 with actin inhibits the actomyosin Mg-ATPase activity. CNN1 is part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1.

Other Names:

Calponin-1, Basic calponin, Calponin H1, smooth muscle

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal [CNN1 (N) (R0197-1)] or the C-terminal [CNN1 (C) (R0197-2)] region of human CNN1. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: