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Quantity: 100 ul                                                                                          Application: WB

Predicted I Observed M.W.: 27 I 32 kDa                                                     Uniprot ID: O23252

Background:

Eukaryotic translation initiation factor 4E-1 (EIF4E1) is a component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. EIF4E1 recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Other Names:

Eukaryotic translation initiation factor 4E-1, eIF-4E-1, eIF4E-1, Protein cucumovirus multiplication 1, eIF-4F 25 kDa subunit, eIF-4F p26 subunit, mRNA cap-binding protein, CUM1

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of arabidopsis thaliana EIF4E1 (At4g18040). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: