Products

R3417-1 - RAD51 (N) Antibody, Rabbit Polyclonal

Catalog No. R3417-1
Product Name RAD51 (N) Antibody, Rabbit Polyclonal
Price $349
  
 

Quantity: 100 ul                                                                                          Application: WB

Predicted I Observed M.W.: 37 kDa                                                            Uniprot ID: P94102


Background:

RAD51 binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 is a component of the meiotic recombination pathway. RAD51 seems to play a role in mediating chromosome homology search, chromosome pairing and synapsis at early stages and probably chromosome crossing-over at later stages in meiosis. RAD51 is probably involved in the repair of meiotic double strand breaks (DBSs) generated by AtSPO11-1 and in homologous recombination. Its function is dispensable for vegetative growth and root mitosis.

Other Names:

DNA repair protein RAD51 homolog 1, Rad51-like protein 1, AtRAD51, At5g20850, F22D1.20

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of arabidopsis thaliana RAD51 (AT5G20850). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

Fig 1. Western blot analysis of total protein extracts
from wild type arabidopsis leaves, untreated (Unt) or
r-irradiated for 1 h (IR); using anti-RAD51 (N) (R3417-1)
at RT for 2 h. The same filter was re-probed with an
non-relevant Ab for the loading control.