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Quantity: 100 ul                                                                                        Application: WB

Predicted I Observed M.W.: 103 I 103, 120, 150 kDa                                 Uniprot ID: Q9ZVD5

Background:

AGO4 is a member of a class of PAZ/PIWI domain containing proteins involved in small interfering RNA (siRNA)-mediated gene silencing. AGO4 is a component of the RISC complex that associate with the siRNA pathway involved in direct cytosine methylation at endogenous DNA repeats. AGO4 forms a AGO4/NRPE1/siRNA complex in cajal body, facilitating its function in RNA-directed gene silencing of target loci. AGO4 is also required for CpNpG and asymmetric DNA methylation as well as histone H3 'Lys-9' methylation (H3K9me) at SUP and SN1 loci. Loss of function mutations have reduced site specific CpNpG and CpHpH methylation and increased susceptibility to bacterial pathogens.

Other Names:

ARGONAUTE 4, OCP11, OVEREXPRESSOR OF CATIONIC PEROXIDASE 11

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of arabidopsis thaliana AGO4 (At2g27040). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: