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Quantity: 100 ul                                                                                        Application: WB

Predicted I Observed M.W.: 36 I 53, 66 kDa                                              Uniprot ID: Q8S307

Background:

BRASSINAZOLE-RESISTANT 1 (BZR1) is a transcriptional repressor that binds to the brassinosteroid (BR) response element (BRRE) 5'-CGTG(T/C)G-3' in gene promoter. BZR1 also positively regulates the brassinosteroid-signaling pathway by mediating downstream growth responses and negative feedback regulation of brassinosteroid biosynthesis. BZR1 promotes growth, and modulates ovule initiation and development by monitoring the expression of genes related to ovule development (e.g. HLL, ANT, and AP2). There is evidence for phosphorylation-dependent nucleocytoplasmic shuttling of BZR1. GSK3-like kinases (including BIN2), 14-3-3 proteins, and the phosphatase BSU1 seem to participate in this process. Phosphorylation also appears to affect BZR1's transcriptional activities.

Other Names:

BRASSINAZOLE-RESISTANT 1, Protein BIN2 SUBSTRATE 2, BIS2                     

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the -terminal region of arabidopsis thaliana BZR1 (AT1G75080). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana 

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: