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R3390-1 - PR1 (N) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB
Predicted I Observed M.W.: 18 kDa Uniprot ID: P33154 Background: PR1 gene expression is induced in response to a variety of pathogens. It is a useful molecular marker for the SAR response. Though the Genbank record for the cDNA associated to this gene is called 'PR-1-like', the sequence actually corresponds to PR1. Expression of this gene is salicylic-acid inducible. Other Names: Pathogenesis-related protein 1, At2g14610, T6B13.15, ATPR1 Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of arabidopsis thaliana PR1 (AT2G14610). Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Arabidopsis thaliana Tested Applications: WB: 1:500-1:2,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. A) Western blot analysis of equal amounts of protein extracts from wild typearabidopsis leaves without (-) or with (+) 1mM salicylic acid (SA) treatment for 17 hr;using anti-PR1 (N) (R3390-1) at RT for 2 h. SA treatment is known to induce PR1 protein.B) Same as in A except that protein extracts from human 293T cells transfected with avector or Flag-PR1 were used.
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