Products

R3483-1 - AXR1 (N) Antibody, Rabbit Polyclonal

Catalog No. R3483-1
Product Name AXR1 (N) Antibody, Rabbit Polyclonal
Price $349
  
 

Quantity: 100 ul                                                                                       Application: WB

Predicted I Observed M.W.: 60 I 60, 55 kDa                                             Uniprot ID: P42744


Background:

AXR1 encodes a subunit of the RUB1 activating enzyme that regulates the protein degradation activity of Skp1-Cullin-Fbox complexes, primarily, but not exclusively, affecting auxin responses. AXR1 acts alongside AS1 to exclude BP expression from leaves.

Other Names:

NEDD8-activating enzyme E1 regulatory subunit, Auxin-resistance protein AXR1, At1g05180, YUP8H12.21, AUXIN RESISTANT 1

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of arabidopsis thaliana AXR1 (AT1G05180). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

 AXR1 Antibody Western (Abiocode)

Fig 1. Western blot of protein extracts from
arabidopsis seeds; using anti-AXR1 (N) (R3483-1)
at RT for 2 h.  The 55 kD and 60 kD bands may
be 2 isoforms of AXR1.