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Quantity: 100 ul                                                                                       Application: WB

Predicted I Observed M.W.: 82, 58, 55, 29 kDa                                        Uniprot ID: O04425

Background:

FCA plays a major role in the promotion of the transition of the vegetative meristem to reproductive development. FCA plays a role in the regulation of flowering time in the autonomous flowering pathway by decreasing FLOWERING LOCUS C mRNA levels. FCA required for RNA-mediated chromatin silencing of a range of loci in the genome. Cotranscriptionally recognizes aberrant RNA and marks it for silencing. Controls alternative cleavage and polyadenylation on pre-mRNAs and antisense RNAs. FCA acts redundantly with FPA to prevent the expression of distally polyadenylated antisense RNAs at the FLC locus.

Other Names:

Flowering time control protein FCA, At4g16280, At4g16290, At4g16300, dl4180c, dl4181c, dl4182c, FCAALL.331

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of arabidopsis thaliana FCA (AT4G16280). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.