Products

R3412-3 - EIN3 (N) Antibody, Rabbit Polyclonal

Catalog No. R3412-3
Product Name EIN3 (N) Antibody, Rabbit Polyclonal
Price $349
  
 

Quantity: 100 ul                                                                                 Application: WB

Predicted I Observed M.W.: 71 I 80, 100 kDa                                     Uniprot ID: O24606


Background:

EIN3 is a probable transcription factor acting as a positive regulator in the ethylene response pathway. EIN3 is required for ethylene responsiveness in adult plant tissues. EIN3 binds to a primary ethylene response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter with consequence to activate the transcription of this gene.

Other Names:

Protein ETHYLENE INSENSITIVE 3, At3g20770, MOE17.8, ATEIN3

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of arabidopsis thaliana EIN3 (At3g20770). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

    EIN3 Antibody Western (Abiocode)            

Fig 1. A) Western blot of protein extracts from human 293T cells transfected with a vector control (Vec) or Flag-EIN3, using indicated Abs at RT for 2 h. B) Western blot of protein extracts from WT arabidopsis grown in the absence (-) or presence of 0.1 mM ACC (1-aminocyclopropane-1-carboxylic acid, the ethylene precursor) for  7 h and 16 h, using anti-EIN3 (N) (R3412-3) at RT for 2 h.