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Quantity: 100 ul                                                                                      Application: WB

Predicted I Observed M.W.: 118, 121 I 120, 150 kDa                                Uniprot ID: Q9C5U0

Background:

AHK4 is a cytokinins (CK) receptor related to bacterial two-component regulators. AHK4 binds also the synthetic urea-type cytokinin thiadiazuron, a potent defoliant and herbicide. AHK4 functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. AHK4 undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. In the presence of cytokinin, AHK4 feeds phosphate to phosphorelay-integrating histidine phosphotransfer protein (HPt) and activates subsequent cascade. In the absence of cytokinin, AHK4 removes phosphate from HPt proteins, decreasing the system phosphoload. AHK4 is involved in meristems establishment in seedlings. AHK4 acts as a redundant negative regulator of drought and salt stress responses, and abscisic acid (ABA) signaling in a cytokinin-dependent manner. AHK4 is required to set vascular asymmetric cell divisions that establish phloem and procambium cell lines. AHK4 is a redundant positive regulator of cytokinin signaling that regulates many development process including seed germination, cell division, seed size, chlorophyll retention during leaf senescence, root repression and shoot promotion.

Other Names:

Histidine kinase 4, Arabidopsis histidine kinase 4, AtHK4, Cytokinin receptor CYTOKININ RESPONSE 1, AtCRE1, Cytokinin receptor CRE1, Phosphoprotein phosphatase AHK4, Protein AUTHENTIC HIS-KINASE 4, Protein ROOT AS IN WOL 1, Protein WOODEN LEG, CRE1, RAW1, WOL, At2g01830, T23K3.2

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of arabidopsis thaliana AHK4 (AT2G01830). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: