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Quantity: 100 ul                                                                                    Application: WB

Predicted I Observed M.W.: 22 kDa                                                      Uniprot ID: Q5Q9J1

Background:

MADS-box protein encoded by FLOWERING LOCUS C (FLC) is a transcription factor that functions as a repressor of floral transition and contributes to temperature compensation of the circadian clock. The expression of FLC is downregulated during cold treatment. Vernalization, FRI and the autonomous pathway all influence the state of FLC chromatin. Both maternal and paternal alleles are reset by vernalization, but their earliest activation differs in timing and location. Histone H3 trimethylation at lysine 4 and histone acetylation are associated with active FLC expression, whereas histone deacetylation and histone H3 dimethylation at lysines 9 and 27 are involved in FLC repression. Expression is also repressed by two small RNAs (30- and 24-nt) complementary to the FLC sense strand 3' to the polyA site. The small RNAs are most likely derived from an antisense transcript of FLC.

Other Names: 

AGAMOUS-LIKE 25, AGL25, FLF, FLOWERING LOCUS C, FLOWERING LOCUS F, REDUCED STEM BRANCHING 6, RSB6 

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of arabidopsis thaliana FLC (At5g10140). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity: 

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: