Products

R3572-1 - FRO2 (C) Antibody, Rabbit Polyclonal

Catalog No. R3572-1
Product Name FRO2 (C) Antibody, Rabbit Polyclonal
Price $349
  
 

Quantity: 100 ul                                                                                    Application: WB

Predicted I Observed M.W.: 82 kDa                                                      Uniprot ID: P92949


Background:

Ferric reduction oxidase 2 (FRO2) is a Flavocytochrome that transfers electrons across the plasma membrane to reduce ferric iron chelates to form soluble ferrous iron in the rhizosphere. FRO2 may be involved in the delivery of iron to developing pollen grains. FRO2 acts also as a copper-chelate reductase, and is involved in glycine betaine-mediated chilling tolerance and reactive oxygen species accumulation.

Other Names:

Ferric reduction oxidase 2, AtFRO2, Ferric-chelate reductase 2, Protein FERRIC CHELATE REDUCTASE DEFECTIVE 1, FRD1

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of arabidopsis thaliana FRO2 (AT1G01580). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

 FRO2 Antibody Western (Abiocode)

Fig 1. A) Western blot of total protein extracts from arabidopsis leaves harvested  8 h or
22 h post light stimulation under the long-day photoperiod condition, using anti-FRO2 (C)
(R3572-1) at RT for 2 h. FRO2 is known to be  regulated diurnally (Vert et al., 2003, Plant
Physiol., 132:796-804). B) Same as in A except that arabidopsis leaves were treated
without (-) or with 1 mM salicylic acid (SA) or 20 uM of Benzyladenine (BA) for 17 h. FRO2
is known to be down-regulated by cytokinins (Seguela et al., 2008, Plant J., 55:289-300).