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Quantity: 100 ul                                                                                        Application: WB

Predicted I Observed M.W.: 30 kDa                                                          Uniprot ID: Q9SQR3

Background:

DWARF14/D14 (Strigolactone esterase D14) is involved in strigolactone signaling pathway. DWARF14/D14 is rapidly degraded in the presence of strigolactones and functions downstream of strigolactone synthesis, as a component of hormone signaling and as an enzyme that participates in the conversion of strigolactones to the bioactive form. DWARF14/D14 acts probably as a strigolactone receptor. The hydrolysis of strigolactone into a covalently linked intermediate molecule initiates a conformational change of DWARF14/D14 to facilitate interaction with MAX2 and formation of the D14-MAX2-SKP1/ASK1 complex to trigger strigolactone signaling. This mechanism defines DWARF14/D14 as a non-canonical hormone receptor with dual functions to generate and sense the active form of strigolactone.

Other Names:

Strigolactone esterase D14, D14, AtD14, At3g03990

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing C-terminal region of arabidopsis thaliana DWARF14 (At3g03990). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: