Quantity: 100 ul Application: WB
Predicted M.W.: 60 kDa Uniprot ID: Q16875
Background:
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase isoform3 (PFKFB3) is a major driver of aerobic glycolysis involved in the synthesis and degradation of fructose 2,6-bisphosphate. Recent studies indicate that IKKβ directly interacts with and phosphorylates PFKFB3 at Ser269 upon glutamine deprivation to inhibit its activity, thereby down-regulating aerobic glycolysis when glutamine levels are low (Reid et al., 2016, Genes Dev., 30:1837-1851).
Other Names:
6PF-2-K, Fru-2,6-P2ase 3, PFK, FBPase 3, NY-REN-56, iPFK-2
Source and Purity:
Rabbit polyclonal antibodies were produced by immunizing animals with the synthetic phosphor-peptide LGGRIGGD-pS-GLSSR corresponding to the amino acids 261-274 of human PFKFB3 protein containing phosphorylated Ser269. Antiserum was purified by a two-step affinity purification procedure to obtain antibody that specifically recognizes the Ser269 phosphorylated PFKFB3 protein.
Storage Buffer and Condition:
Supplied in 1 x PBS (PH7.4), 100ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.
Species Specificity:
Human
Tested Applications:
WB: 1:1,000-1:3,000 (detect endogenous protein*)
*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.
Product Data:
See experimental data described in Reid et al., 2016, Genes Dev., 30:1837-1851.
Reference:
1. Reid MA, Lowman XH, Pan M, Tran TQ, Warmoes MO, Ishak Gabra MB, Yang Y, Locasale JW, Kong M. IKKβ promotes metabolic adaptation to glutamine deprivation via phosphorylation and inhibition of PFKFB3. Genes Dev., 30:1837-1851, 2016.