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R3355-1 - MSLN (N) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB Predicted I Observed M.W.: 71 I 75kD Uniprot ID: Q13421
Background: Mesothelin (MSLN) is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. The MSLN gene encodes a 71 kDa precursor protein that is processed into two mature proteins: the N-terminal Megakaryocytic Potentiating Factor (MPF) and the C-terminal mature MSLN (mMSLN). MPF is a 31 kDa glycoprotein that is secreted from the cell into the blood. The mMSLN is a 40 kDa glycoprotein protein that remains bound to the cell membrane by phosphatidyl inositol. Other Names: Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, Megakaryocyte-potentiating factor, MPF Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human MSLN protein. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human Tested Applications: WB: 1:500-1:2,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. Western blot analysis of equal amounts of protein extractsfrom HeLa cells (the negative control) and human IGROVE1 ovariancancer cells, using anti-MSLN(N) (R3355-1) at RT for 2 h.
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