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Predicted M.W.: 44 kDa                                                                    Uniprot ID: P08727

Application: WB

Quantity:        50 ul KRT19 (C) (R0145-1) Rabbit Polyclonal Antibody &

                      50 ul KRT19 (N) (R0145-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Keratin 19 (KRT19) is a member of the keratin family.  Keratins are intermediate filament proteins in charge of maintaining the structural integrity of epithelial cells.  They are subdivided into cytokeratins and hair keratins.  KRT19 plays a role in the organization of myofibers.  It works with KRT8, linking the contractile apparatus to dystrophin at the costameres of striated muscle.   

Other Names:

CK19, CK-19, K19, K1CS, MGC15366, Cytokeratin-19

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the C-terminal [KRT19 (C) (R0145-1)] or the N-terminal [KRT19 (N) (R0145-2)] regions of human KRT19.  Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications: 

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: