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Predicted M.W.: 20 kDa                                                                    Uniprot ID: Q9GZX6

Application: WB, ELISA

Quantity:        50 ug IL-22 (N) (M0004-1) Mouse Monoclonal Antibody &

                      50 ug IL-22 (C) (R0281-1) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

IL-22 is a member of the IL-10 cytokine superfamily, which are potent mediators of cellular inflammatory responses.  IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens, especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 along with IL-17 is rapidly produced by splenic LTi-like cells and can also be produced by Th17 cells. IL-22 plays a role in the coordinated response of both adaptive and innate immune systems. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the co-expressed cytokine IL-17A.

Other Names:

IL-10-related T-cell-derived-inducible factor, IL-TIF, ZCYTO18, UNQ3099/PRO10096

Source and Purity:

Moue monoclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human IL-22. Antibodies were purified by Caprylic Acid- Ammonium Sulfate precipitation. Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of human IL-22. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 40% Glycerol, 0.02% Thimerosal  (M0004-1) or 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃ (R0281-1). Store at -20 °C. Stable for at least 6 months from date of receipt.

Species Specificity:

Human

Tested Applications:

WB: 1:1,000-1:5,000 (detect recombinant protein)

ELISA:  1:1,000- 1:10,000 (detect native recombinant protein in a direct ELISA assay). 

              Suitable for using as ELISA capture or detection antibodies.

Product Data: