Abiocode Logo

Uniprot ID: P07900                                                                                   Application: WB

Predicted | Observed MW: 85 | 90 kDa                                                      

Quantity:        50 ul HSP90AA1 (N2) (R0515-4) Rabbit Polyclonal Antibody &

                      50 ul HSP90AA1 (C) (R0515-5) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Heat shock protein HSP 90-alpha (HSP90AA1) is a molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved in cell cycle control and signal transduction. HSP90AA1 undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the target proteins, thereby causing their activation. HSP90AA1 interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

Other Names:

Heat shock protein HSP 90-alpha, Heat shock 86 kDa, HSP 86, Renal carcinoma antigen NY-REN-38, HSP90A, HSPC1, HSPCA

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [HSP90AA1 (N2) (R0515-4)] or the C-terminal [HSP90AA1 (C) (R0515-5)] region of human HSP90AA1. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: