Abiocode Logo
Products
Contact Us
- Telephone:
1-818-707-0309 - E-Mail:
Abiocode@Abiocode.com
R1062-vp - TDG (vPairTM) Antibodies
|
Predicted M.W.: 46 kDa Uniprot ID: Q13569 Application: WB Quantity: 50 ul TDG (N) (R1062-1) Rabbit Polyclonal Antibody & 50 ul TDG (C) (R1062-2) Rabbit Polyclonal Antibody Product Introduction: vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS. Background: G/T mismatch-specific thymine DNA glycosylase (TDG) belongs to the TDG/mug DNA glycosylase family. TDG removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. TDG also removes thymine from C/T and T/T mispairings with lower activity. In addition, TDG can remove uracil and 5-bromouracil from mispairings with guanine. TDG plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. Other Names: G/T mismatch-specific thymine DNA glycosylase, Thymine-DNA glycosylase Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal [TDG (N] (R1062-1)] or the C-terminal [TDG (C) (R1062-2)] region of human TDG. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human, Mouse Tested Applications: WB: 1:1,000-1:5,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. Western blot of total cell extracts from (A, D) mouse thymocytes, (B, E) human MCF7 and (C, F) human Jurkat, using 2 independent antibodies against 2 distinct regions of human TDG at RT for 2 h. The observed M.W. of TDG is approximately 56 kD. The appearance of TDG as doublets has been reported by Cortellino et al. (Cell, 2011, 146:67). The potentially sumoylated TDG indicated by * has been described by Hardeland et al. (EMBO, 2002, 21:1456). |