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Predicted | Observed MW: 153 | 180 kDa                                                   Uniprot ID: Q07890

Application: WB

Quantity:        50 ul SOS2 (M) (R1188-1) Rabbit Polyclonal Antibody &

                      50 ul SOS2 (C) (R1188-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Son of Sevenless (SOS) was discovered in Drosophila melanogaster. SOS has two human homologues, SOS1 and SOS2. SOS proteins are guanine-nucleotide releasing factor that can promote the exchange of Ras-bound GDP by GTP to activate Ras in response to growth factor stimulation. SOS proteins also appear to serve as a guanine nucleotide exchanger for Rac and, thus, may be involved in cytoskeleton reorganization. SOS proteins may be recruited to different cellular locations through interactions with the adaptor proteins Grb2 and E3b1.

Other Names:

Son of sevenless homolog 2, SOS-2

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the middle [SOS2 (M) (R1188-1)] or the C-terminal [SOS2 (C) (R1188-2)] region of human SOS2. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: