Products

R0171-vp - ZHX2 (vPairTM) Antibodies

Catalog No. R0171-vp
Product Name ZHX2 (vPairTM) Antibodies
Price $249
  
 

Predicted M.W.: 92 kDa                                                                    Uniprot ID: Q9Y6X8

Application: WB

Quantity:        50 ul ZHX2 (N) (R0171-1) Rabbit Polyclonal Antibody &

                       50 ul ZHX2 (N2) (R0171-2) Rabbit Polyclonal Antibody


Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Zinc fingers and homeoboxes protein 2 (ZHX2) is a member of the zinc fingers and homeoboxes gene family, which are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. In addition to forming homodimers, ZHX2 interacts with ZHX1 and ZHX3 to form heterodimers.

Other Names:

Alpha-fetoprotein regulator 1, AFP regulator 1, Regulator of AFP, AFR1, KIAA0854, RAF

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing 2 distinct  N-terminal regions of human ZHX2. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications: 

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

ZHX2 Antibody Western (Abiocode)

Fig 1. Western blot of total cell extracts from
(A, C) human HepG2, (B) human HeLa, using
2 independent Abs against 2 distinct N-terminal
regions of human ZHX2 at RT for 2 h.