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Uniprot ID: P16382                                                                                    Application: WB

Predicted | Observed MW: 88 kDa                                                             

Quantity:        50 ul IL4R (N1) (R0595-1) Rabbit Polyclonal Antibody &

                      50 ul IL4R (N3) (R0595-3) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Interleukin-4 receptor subunit alpha (IL4R) is a single-pass type I membrane protein belonging to the type I cytokine receptor family and the Type 4 subfamily. IL4R is a receptor for both interleukin 4 and interleukin 13. IL4R couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation. In certain cell types, IL4R can signal through activation of insulin receptor substrates, IRS1/IRS2.

Other Names:

Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, CD124, Il4ra

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing 2 distinct N-terminal regions of mouse IL4R. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Mouse

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: