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Predicted M.W.: 183 kDa                                          Uniprot ID: P26358

Application: WB              

Quantity:       50 ul DNMT1 (C) (R0020-1) Rabbit Polyclonal Antibody &

                      50 ul DNMT1 (N) (R0020-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

DNA (cytosine-5)-methyltransferase 1 (DNMT1) plays a role in methylating CpG residues. Preferentially methylates hemimethylated DNA and regulates tissue-specific patterns of methylated cytosine residues.  During S phase of the cell cycle DNMT1 localizes at replication sites conserving methylation patterns in the newly synthesized strand, maintaining epigenetic inheritance.   During G2 and M phases of the cell cycle, DNMT1 associates with chromatin maintaining DNA methylation irrespective of replication. Atypical DNA methylation patterns have been shown to be associated with certain human tumors and developmental abnormalities.  

Other Names:

Cxxc9, Dnmt, Dnmt1o, MTase, Met-1, Met1, MommeD2

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal [DNMT1 (N) (R0020-2)] or the C-terminal region [DNMT1 (C) (R0020-1)] of human DNMT1. Antibodies were purified by affinity purification using immunogens. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: