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Predicted M.W.: 85 kDa                                                        Uniprot ID: P35222

Application: WB

Quantity:          50 ul b-catenin1 (N) (R0008-1) Rabbit Polyclonal Antibody &

                          50 ul b-catenin1 (C) (R0008-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

β-catenin1 is a key downstream effector of the Wnt signaling pathway. It is implicated in early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin1 on Ser45, which primes β-catenin1 for subsequent phosphorylation by GSK-3 at Ser33, Ser37 and Thr41. Phosphorylation of β-catenin1 by GSK-3β leads to its degradation. Wnt signaling antagonizes GSK-3b and stablizes b-catenin1. Mutations in these phosphorylation sites, which result in the stabilization of β-catenin1 protein levels, have been found in many tumor cell lines.

Other Names:

beta-catenin, CTNNB1, OK/SW-cl.35, CATNB, DKFZp686D02253, FLJ25606, FLJ37923

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal [b-catenin1 (N) (R0008-1)] or the C-terminal region [b-catenin1 (C) (R0008-2)] of human b-catenin1. Antibodies were purified by affinity purification using immunogens. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: