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Predicted I Observed M.W.: 84 kDa                                                            Uniprot ID: Q02156

Application: WB

Quantity:        50 ul PRKCE (N) (R2264-1) Rabbit Polyclonal Antibody &

                      50 ul PRKCE (C) (R2264-2) Rabbit Polyclonal Antibody 

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

PRKCE, protein kinase C epsilon type, is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PRKCE phosphorylates a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PRKCE also serves as major receptors for phorbol esters, a class of tumor promoters. PRKCE has also been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.

Other Names:

nPKC-epsilon, PKCE 

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal [PRKCE (N) (R2264-1)] or the C-terminal [PRKCE (C) (2264-2)] region of human PRKCE. Antibodies were purified by affinity purification using immunogen. 

Species Specificity:

Human, Mouse 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt. 

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: