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Quantity: 100 ul                                                                                Application: WB, IF

Predicted I Observed M.W.: 183 I 220 kDa                                        Uniprot ID: P26358

Background:

DNA (cytosine-5)-methyltransferase 1 (DNMT1) plays a role in methylating CpG residues. Preferentially methylates hemimethylated DNA and regulates tissue-specific patterns of methylated cytosine residues.  During S phase of the cell cycle DNMT1 localizes at replication sites conserving methylation patterns in the newly synthesized strand, maintaining epigenetic inheritance.   During G2 and M phases of the cell cycle, DNMT1 associates with chromatin maintaining DNA methylation irrespective of replication. Atypical DNA methylation patterns have been shown to be associated with certain human tumors and developmental abnormalities.  

Other Names:

Cxxc9, Dnmt, Dnmt1o, MTase, Met-1, Met1, MommeD2

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human DNMT1. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IF (Immunofluorescence): 1:100-1:300

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: