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R3180-1 - SPO11 (C) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB, IP Predicted I Observed M.W.: 45 I 48 kDa Uniprot ID: Q9Y5K1 Background: Meiotic recombination protein SPO11 is a nuclear protein that belongs to the TOP6A family. SPO11 is required for meiotic recombination. SPO11 mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. Other Names: Meiotic recombination protein SPO11, Cancer/testis antigen 35, CT35 Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of human SPO11. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human, Mouse Tested Applications: WB: 1:1,000-1:3,000 (detect endogenous protein*) IP: 1:100-1:200
*: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. A) Western blot of total cell extracts from mouse thymus; using 2 independent Abs against 2 distinct regions of human SPO11 at RT for 2 h. B) Total extracts from mouse thymus were immunoprecipitated (IP) with IgG or anti-SPO11 (C) (R3180-1); followed by WB with the same Ab and Rabbit IgG light chain-specific 2nd antibody. |