Products

R3180-1 - SPO11 (C) Antibody, Rabbit Polyclonal

Catalog No. R3180-1
Product Name SPO11 (C) Antibody, Rabbit Polyclonal
Price $249
  
 

Quantity: 100 ul                                                                                          Application: WB, IP

Predicted I Observed M.W.: 45 I 48 kDa                                                     Uniprot ID: Q9Y5K1


Background:

Meiotic recombination protein SPO11 is a nuclear protein that belongs to the TOP6A family. SPO11 is required for meiotic recombination.  SPO11 mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination.

Other Names:

Meiotic recombination protein SPO11, Cancer/testis antigen 35, CT35

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of human SPO11. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP: 1:100-1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

Fig 1. A) Western blot of total cell extracts from mouse thymus; using 2
independent Abs against 2 distinct regions of human SPO11 at RT for 2 h.
B) Total extracts from mouse thymus were immunoprecipitated (IP) with
IgG or anti-SPO11 (C) (R3180-1); followed by WB with the same Ab and
Rabbit IgG light chain-specific 2nd antibody.