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Predicted I Observed M.W.: 45 I 48 kDa                                                     Uniprot ID: Q9Y5K1

Application: WB, IP

Quantity:        50 ul SPO11 (C) (R3180-1) Rabbit Polyclonal Antibody &

                      50 ul SPO11 (N) (R3180-2) Rabbit Polyclonal Antibody 

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Meiotic recombination protein SPO11 is a nuclear protein that belongs to the TOP6A family. SPO11 is required for meiotic recombination.  SPO11 mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination.

Other Names:

Meiotic recombination protein SPO11, Cancer/testis antigen 35, CT35

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the C-terminal [SPO11 (C) (R3180-1)] or the N-terminal [SPO11 (N) (R3180-2)] region of human SPO11. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP: 1:100-1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: