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Quantity: 100 ul                                                                                         Application: WB

Predicted I Observed M.W.: 27, 46 I 27 kDa                                               Uniprot ID: Q86XR7

Background:

TIR domain-containing adapter molecule 2 (TICAM2) functions as sorting adapter in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. TICAM2 physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1; signaling is proposed to occur in early endosomes after endocytosis of TLR4. TICAM2 may also be involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB; however, reports are controversial. Two isoforms of TICAM2 have been identified: isoform 1 (also known as TRAM) and isoform 2 (also known as TAG; TRAM adaptor with GOLD domain), which differ at their N-terminus. Isoform 2 is proposed to inhibit LPS-TLR4 signaling at the late endosome by interaction with isoform 1; thereby disrupting the association of isoform 1 with TICAM1.

Other Names:

TIR domain-containing adapter molecule 2, TICAM-2, Putative NF-kappa-B-activating protein 502,
TRIF-related adapter molecule, Toll-like receptor adaptor protein 3, Toll/interleukin-1 receptor domain-containing protein, MyD88-4, TIRAP3, TIRP, TRAM

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing middle region of human TICAM2. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: