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Quantity: 100 ul                                                                                                       Application: WB

Predicted | Observed MW: 51 kDa                                                                    Uniprot ID: Q01543

Background:

Friend leukemia integration 1 (FLI1) is a transcription factor containing an ETS DNA-binding domain. FLI1 is a sequence-specific transcriptional activator that recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. The FLI1 gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants [provided by RefSeq].

Other Names:

Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human FLI1. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

Fig 1. Western blot of total cell extracts
from mouse thymus; using anti-FLI1 (N)
(R2553-1) at RT for 2 h.