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R2401-vp - AFF3 (vPairTM) Antibodies
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Predicted I Observed M.W.: 133 I 170 kDa Uniprot ID: P51826 Application: WB Quantity: 50 ul AFF3 (N) (R2401-1) Rabbit Polyclonal Antibody & 50 ul AFF3 (C) (R2401-2) Rabbit Polyclonal Antibody Product Introduction: vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS. Background: AF4/FMR2 family member 3 (AFF3) belongs to the AF4 family and is a putative transcription activator that may function in lymphoid development and oncogenesis. AFF3 binds, in vitro, to double-stranded DNA. Other Names: AF4/FMR2 family member 3, LAF4, LAF-4, Lymphoid nuclear protein related to AF4 Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [AFF3 (N) (R2401-1)] or the C-terminal [AFF3 (C) (R2401-2)] region of human AFF3. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human, Mouse Tested Applications: WB: 1:1,000-1:3,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. Western blot of total cell extracts fromA, D) human HeLa, B, E) human Jurkat, C) mouse thymus; using 2 independent Abs against 2 distinct regions of human AFF3 at RT for 2 h. |