R3451-1 - MPK6 (N) Antibody, Rabbit Polyclonal

Catalog No. R3451-1
Product Name MPK6 (N) Antibody, Rabbit Polyclonal
Price $349

Quantity: 100 ul                                                                                    Application: WB

Predicted I Observed M.W.: 45 I 48 kDa                                               Uniprot ID: Q39026


MITOGEN-ACTIVATED PROTEIN KINASE 6 (MPK6) is involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. MPK6 may be involved in hypersensitive response (HR)-mediated signaling cascade by modulating LIP5 phosphorylation and subsequent multivesicular bodies (MVBs) trafficking. MPK6 may phosphorylate regulators of WRKY transcription factors. MPK6 regulates locally gene-mediated and basal resistance response to certain pathogens. MPK6 may be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MKK1/MKK2 and MPK4/MPK6). MKK1-MPK6 module mediates abscisic acid (ABA)-dependent CAT1 expression with H2O2 production and response to drought and salt stress. MKK1-MPK6 module is also involved in sugar signaling during the process of seed germination. MKK3-MPK6 module plays an important role in the jasmonate signal transduction pathway through the negative regulation of MYC2/JIN1 expression. MKK9-MPK3/MPK6 module phosphorylates and activates EIN3, leading to the promotion of EIN3-mediated transcription in ethylene signaling. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs.

Other Names:

AtMAPK6, MPK6, At2g43790

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of arabidopsis thaliana MPK6 (AT2G43790). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Tested Applications:  

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Species Specificity:

Arabidopsis thaliana

Product Data:

MPK6 Antibody Western (Abiocode)

Fig 1. Western blot analysis of equal amounts of protein extracts from wild type (WT) and Mpk6 deficient mutant (Mut) arabidopsis leaves, using anti-MPK6(N) (R3451-1) at RT for 2 hr.