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Quantity: 100 ul                                                                                         Application:  WB

Predicted M.W.: 62 kDa                                                                             Uniprot ID: P10820

Background:

PERFORIN (PRF1) is a cytolytic protein found in the granules of CD8 T-cells and NK cells. Upon degranulation, PERFORIN inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of PERFORIN is the MACPF domain, which shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. PERFORIN has structural and functional similarities to complement component 9 (C9). Like C9, PERFORIN creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. PERFORIN is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in PERFORIN cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood.

Other Names:

Cytolysin, Lymphocyte pore-forming protein, P1, PRF1, PFP

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the middle region of mouse PERFORIN.  Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:5,000 (detect recombinant protein)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: