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Quantity: 100 ul                                                                                             Application: WB

Predicted M.W.: 46 kDa                                                                                Uniprot ID: Q13569

Background:

G/T mismatch-specific thymine DNA glycosylase (TDG) belongs to the TDG/mug DNA glycosylase family. TDG removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. TDG also removes thymine from C/T and T/T mispairings with lower activity. In addition, TDG can remove uracil and 5-bromouracil from mispairings with guanine. TDG plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine.

Other Names:

G/T mismatch-specific thymine DNA glycosylase, Thymine-DNA glycosylase

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human TDG. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: