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R2249-2 - ZSCAN10 (C) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB, IP Predicted I Observed M.W.: 80 I 100 kDa Uniprot ID: Q96SZ4 Background: ZSCAN10, zinc finger and SCAN domain-containing protein 10 is required to maintain ES cell pluripotency, and can both activate and /or repress expression of target genes, depending on the context. Specifically binds the 5'-[GA]CGCNNGCG[CT]-3' DNA consensus sequence. ZSCAN10 regulates expression of POU5F1/OCT4, ZSCAN4 and ALYREF/THOC4. Other Names: Zinc finger protein 206, ZNF206 Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of human ZSCAN10. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human, Mouse Tested Applications: WB: 1:1,000-1:3,000 (detect endogenous protein*) IP: 1:100-1:200
*: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. (A) Western blot of total cell extracts from a. mouse thymus, b. human HeLa; using anti-ZSCAN10 (C) (R2249-2) at RT for 2 h. (B) IP-WB. Immunoprecipitation (IP) was performed with human HeLa extracts with IgG or anti-ZSCAN10 (C) (R2249-2), followed by WB with the same Ab. |