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Quantity: 100 ul                                                                                          Application: WB

Predicted I Observed M.W.: 44 I 48 kDa                                                     Uniprot ID: Q13686

Background:

ALKBH1, alkylated DNA repair protein alkB homolog 1, repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. ALKBH1 requires molecular oxygen, alpha-ketoglutarate and iron. ALKBH1 may have a role in placental trophoblast lineage differentiation. ALKBH1 has DNA lyase activity and introduces double-stranded breaks at abasic sites. ALKBH1 cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites.

Other Names:

Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA lyase ABH1, DNA oxidative demethylase ALKBH1, ABH, ABH1, ALKBH

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human ALKBH1. Antibodies were purified by affinity purification using immunogen.

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications. 

Product Data: