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Predicted I Observed M.W.: 107 kDa                                                          Uniprot ID: Q6ZMT4

Application: WB, IP

Quantity:        50 ul KDM7 (M) (R0234-1) Rabbit Polyclonal Antibody &

                        50 ul KDM7 (C) (R0234-2b) Rabbit Polyclonal Antibody

Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Lysine-specific histone demethylase 7 (KDM7) is a histone demethylase required for brain development. KDM7 specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. KDM7 specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2.

Other Names:

Lysine-specific demethylase 7, JmjC domain-containing histone demethylation protein 1D. JHDM1D, KIAA1718

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the middle [KDM7 (M) (R0234-1)] or the C-terminal [KDM7 (C) (R0234-2b)] region of human KDM7. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP: 1:100 - 1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: