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Predicted M.W.: 21 kDa                                                        Uniprot ID: P07321

Application: WB

Quantity:        50 ul EPO (N) (R0064-1) Rabbit Polyclonal Antibody &

                      50 ul EPO (C) (R0064-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Erythropoietin (EPO) is a member of the EPO/TPO family.  It is a glycosylated cytokine that is involved in the regulation of erythrocyte differentiation and initiating hemoglobin synthesis.  EPO is also thought to have neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types.                                                        

Other Names:

EP, MGC138142, MVCD2 (human)

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal region [EPO (N) (R0064-1)] or the C-terminal region [EPO (C) (R0064-2)] of mouse EPO.  Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Mouse

Tested Applications: 

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: